Endocrine Abstracts

Objective: It is known sex steroids affect fat distribution with men and women. With men there is a tendency to deposit fat abdominally. Men are more likely to have more visceral fat than premenopausal women, with whom the preferential fat distribution is gluteofemoral and the percentage of body fat overall higher. Androgens may affect fat tissue with men either directly by androgen receptor stimulation, or indirectly by oestrogen receptor stimulation after aromatization. Inte...

Although neuroactive steroids are well characterized, their physiological relevance in human pregnancy remains indecisive. Hence, we attempted to derive this information from the combination of our metabolomic data, pharmacological data found in the literature and knowledge contained in gene expression databases. These results indicate that GABAergic steroids influence maternal CNS but have limited effect in the fetal brain and maternal and fetal periphery, partly because of t...

BackgroundRapid induction of eu/hypothyroidism in patients with immune hyperthyroidism has been known to precipitate Thyroid eye disease (TED). Our aim was to investigate the incidence of TED in patients treated with radio-iodine therapy (RIT) at our hospital.MethodsWe examined case notes of 45 patients who underwent radio-iodine therapy for Graves’ disease with 544.7 (95% CI- 522.3 – 567.1) MBq bet...

The LRP5 gene is believed to be primarily associated with bone metabolism via Wnt signalling. The latter pathway, however, seems to control various other systems outside the skeleton. We identified the C/T (c.4037:A1330V) polymorphism in the LRP5 gene using a restriction analysis of the PCR product in a cohort of 165 white untreated pre-and post-menopausal women. In a subset of 84 post-menopausal women we analysed the association between the LRP5 genotype and circulatin...

Introduction: Waist circumference serves as a key indicator of abdominal obesity and predictor of cardiovascular and metabolic risk even in children. Under the current WHO standard methodology, waist circumference is measured midway between the lowest rib and the iliac crest. However, comparison of current and earlier data is complicated as different waist circumference measurement methodologies have been used in the past. For instance, previous Czech studies commonly worked w...

Recently we have completed steroid metabolomic study including unconjugated (n=39) and conjugated steroids (n=30) in maternal and fetal body fluids using GC-MS method with two separate multicomponent analyses (for unconjugated steroids and steroid polar conjugates). Our previous data indicate that primarily oxidative isoforms of pluripotent 17β-hydroxysteroid dehydrogenases and aldoketoreductases in placental tissue close to the fetal compartment convert 3&#...

Recently we demonstrated a close correlation between the gestational age (GA) and steroid metabolome in maternal and fetal body fluids using multivariate models including both unconjugated (n=39) and conjugated steroids (n=30) that were measured by GC-MS method including two separate multicomponent analyses (for unconjugated steroids and steroid polar conjugates). We focused on the comparison of the predictive value of the levels of unconjugated steroids alone, c...

Aim: To investigate total homocysteine (Hcy), cysteine (Cys), glutathione (GSH), lipid and steroid sulfate levels in women with polycystic ovary syndrome (PCOS).Methods: The blood levels of Hcy, Cys, GSH (determined by HPLC), total and HDL-cholesterol, insulin (IRMA),DHEA and DHEAS, cortisol, pregnenolone, 17-hydroxy-pregnenolone and pregnenolone-sulphate (all RIA) were examined in 44 women with PCOS (age 24+/-7yrs) and 12 healthy controls (34+/-5yrs) in...

Aim: To evaluate the effects of cyproteronacetate/ethinylestradiol (Diane 35) on weight, lipid and androgen levels in women with PCOS.Methods: 19 PCOS women, age 24.3 +/- 4.3 yrs, weight (W) 66.3 +/- 13.5 kg. Blood samples in the early follicular phase of menstrual cycle for cholesterol (C), HDL cholesterol (HDL-C), triglycerides (TG), testosterone (T), androstenedione (A), DHEAS and SHBG and again after 7 +/- 3.8 months of treatment with Diane 35.<p...

Objective: Skeletal disability pathogenesis is multifactorial. Many studies have proved a negative relation of smoking on calcium-phosphate metabolisms, bone density and quality; however in some other papers these results have not been confirmed. The aim of the study was to monitor the influence of the length of smoking, a number of cigarettes smoked and the age of the first use of cigarettes on the whole-body bone density.Methods: Whole-body densitometr...